Title | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Bojmar L, Kim HSang, Tobias GC, Vatter FAPelissie, Lucotti S, Gyan KEnnu, Kenific CM, Wan Z, Kim K-A, Kim DA, Hernandez J, Pascual V, Heaton TE, La Quaglia MP, Kelsen D, Trippett TM, Jones DR, Jarnagin WR, Matei IR, Zhang H, Hoshino A, Lyden D |
Journal | STAR Protoc |
Volume | 2 |
Issue | 1 |
Pagination | 100225 |
Date Published | 2021 03 19 |
ISSN | 2666-1667 |
Abstract | We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020). |
DOI | 10.1016/j.xpro.2020.100225 |
Alternate Journal | STAR Protoc |
PubMed ID | 33786456 |
PubMed Central ID | PMC7988237 |
Grant List | P30 CA008748 / CA / NCI NIH HHS / United States UL1 TR002384 / TR / NCATS NIH HHS / United States |